This study examined resource mobilisation and innovative culture of small and medium-scale enterprises (SMEs) owners in South-West, Nigeria. Five research questions were raised. The study adopted the descriptive survey research design. The sample comprised one thousand, four hundred and seventeen (1417) SME owners / managers out of which one thousand, three hundred and eighty-two (1382) responded appropriately, this showed 97% response rate. Data were collected using two instruments; these are Resource Mobilisation Questionnaire (r = 0.85) and Innovative Culture Scale (r = 0.68). Data were analysed using descriptive statistics, Pearson Product Moment Correlation and Independent t test.The results showed that SME owners possessed high level of resource mobilisation and innovative culturein South-West, Nigeria. Significant relationship existed between resource mobilisation and innovative culture of SME Owners’ in South-West, Nigeria. Furthermore, there was significant difference in the score on resource mobilisation of SME Owners while no significant difference existed in the score on innovative culture of SME Owners in South-West, Nigeria. Based on the findings, it was recommended among others that Government agencies should create strategies to improve resource mobilisation. This, in turn, will upgrade and enhance innovation among SMEs.